Instrumental Methods of Analysis Unit 1 Notes PDF: UV–visible spectroscopy and fluorimetry (Sem 7)

B-Pharmers, prepare to unveil the secrets of molecules with light in B Pharm Instrumental Method Of Analysis Unit 1 PDF Notes! We’ll be exploring the world of UV-Visible spectroscopy, where light shines a revealing beam on compounds. Imagine electronic transitions causing molecules to absorb specific colors, creating unique “fingerprints” we can use to identify and measure them. Buckle up as we delve into chromophores, the colorful culprits behind these transitions, and auxochromes, their intensity-boosting buddies. We’ll explore how solvents can even influence the light show, and understand the famous Beer-Lambert Law, the equation that quantifies it all. But it’s not just theory; we’ll peek inside the instruments, dissecting light sources, wavelength selectors, and detectors that capture the light signal. Finally, we’ll put this knowledge to work, exploring applications like spectrophotometric titrations and analyzing mixtures like a pro. Get ready to see the invisible made visible, unlocking the power of light in pharmaceutical analysis!

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B Pharm Instrumental Method Of Analysis Unit 1 PDF Notes

Unit 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications