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B Pharm Instrumental Method Of Analysis PDF Notes

B-Pharmers, are you ready to unleash your inner detective and unlock the secrets of molecules through instruments? Dive into the exciting world of B Pharm Instrumental Method Of Analysis PDF Notes, where powerful tools become your allies in dissecting the composition of everything from medicines to food! Imagine using light, electricity, or even sound waves to identify and measure different components with incredible precision. This unit equips you with the fundamentals of various techniques, like spectroscopy, chromatography, and electrochemistry, each offering a unique window into the molecular world. Get ready to decipher spectra, separate mixtures like a pro, and understand how these methods play a crucial role in quality control, research, and various aspects of the pharmaceutical field. It's time to trade textbooks for beakers and lasers, embarking on a thrilling journey of discovery through the power of instrumental analysis!

B Pharm Instrumental Method Of Analysis Notes
B Pharm Instrumental Method Of Analysis Notes


This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart a fundamental knowledge on the principles and instrumentation of spectroscopic and chromatographic technique. This also emphasizes on theoretical and practical knowledge on modern analytical instruments that are used for drug testing.


Upon completion of the subject student shall be able to

  1. Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis

  2. Understand the chromatographic separation and analysis of drugs.

  3. Perform quantitative & qualitative analysis of drugs using various analytical instruments.

B Pharm Instrumental Method Of Analysis PDF Notes

Unit 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component analysis


Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

Unit 2

IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications

Nepheloturbidometry- Principle, instrumentation and applications

Unit 3

Introduction to chromatography

Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications.

Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.

Paper chromatography-Introduction, methodology, development techniques, advantages, disadvantages and applications

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

Unit 4

Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications

High performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications.

Unit 5

Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications

Gel chromatography- Introduction, theory, instrumentation and applications Affinity chromatography- Introduction, theory, instrumentation and applications

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